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. 2019 Mar 26;11(3):430. doi: 10.3390/cancers11030430

Figure 9.

Figure 9

Effect of STIM1 depletion on CDDP-induced cell death and ROS production in HeLa cells. (A) Bar histogram showing the percentage of cells with low ΔΨm in the presence of 25 µM CDDP in siCTRL or siSTIM1 transfected HeLa cells. Data are means of three experiments ± S.D. Student’s t test was employed to assess statistical significance. ** p < 0.01. (B) Immunoblot analysis showing the effect of 24 h treatment with 25 µM CDDP on PARP-1 cleavage in siCTRL or siSTIM1 transfected cells. GAPDH was used as a loading control. (C) Bar histogram showing the mean level of fluorescence emitted by the H2DCFDA probe allowing ROS production assessment in the presence of 25 µM CDDP in siCTRL or siSTIM1 transfected HeLa cells. Data are means of three experiments ± S.D. Student’s t test was employed to assess statistical significance. *** p < 0.001.