Figure 2.

Survival of organoids under hypothermic conditions with antifreeze glycoprotein (AFGP) and α-lactalbumin present. (a) Survival rates of organoids at the different developmental stages when AFGP was added to the growth medium. Rates were obtained from visually assessing the organoids every 24 h during hypothermic storage periods ranging from 24 to 120 h. Bars show results pooled from different independent experiments. (b) Corresponding survival rates obtained from a visual assessment performed two to three days after the organoids were returned to the incubator. (c) Survival rates of organoids at the different developmental stages when α-lactalbumin was added to the growth medium. Black asterisks denote significant differences between developmental stages, and red asterisks below bars denote differences from controls in Figure 1 (* p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant).