Figure 1.

Proliferation-dependent expression of ANO10 in FRT cells. (A) FRT cells were grown in FCS-containing media at 70% density (Proliferating), as confluent monolayer in FCS-free media (Monolayer), or as polarized monolayer on permeable supports and in FCS-free media (Polarized). Expression of endogenous ANO10 (green fluorescence) was intracellular in dividing cells (Proliferating), but was detected in the plasma membrane and in the primary cilium in densely grown cells (Monolayer). ANO10 was more prominent in plasma membrane and primary cilium in polarized cells (Polarized). For further details and references, see main text. (B) Hypothetical model proposing variable cellular locations of ANO10 depending on cell proliferation or cell polarization. ANO10 is found primarily intracellularly, but is also in the plasma membrane during cell cycle. Reduced expression of ANO10 and translocation into the primary cilium is observed once cells move into G₀. Bar, 20 µm [108].