Figure 1.

Comparison of in vivo anti-infective efficacy of P. guajava leaf extracts prepared by three different extraction methods, against P. aeruginosa ( A– C), and S. aureus ( D– F). Catechin (50 μg/mL) and gentamicin (0.1 μg/mL) employed as positive controls conferred 100% and 80% protection on the worm population, respectively. DMSO present in the ‘vehicle control’ at 0.5%v/v did not affect virulence of the bacterium towards C. elegans. DMSO (0.5%v/v) and GLE at tested concentrations showed no toxicity towards C. elegans. MAE: Microwave Assisted Extraction; VAE: Vacuum Assisted Extraction; GLE: Guava Leaf Extract.