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. 2019 Mar 8;8(3):226. doi: 10.3390/cells8030226

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FeAS shows little influence on the development of myeloid cells, megakaryocytes, and B lymphocytes. (A,B) Myeloid cells, megakaryocytes, and B lymphocytes were induced to develop under the indicated conditions using the OP9 system, and the respective lineage markers, Gr-1, CD41 and B220, were examined by flow cytometry analysis. Reactivity to 7-AAD was also analyzed by flow cytometry. The percentage of each fraction is indicated. (C) The morphology of granulocytes and megakaryocytes treated with FeAS obtained by the above-described culture conditions were analyzed.