Figure 6.

VNLG-152R inhibits TNBC cells metastasis in vitro and in vivo. (A) Real-time invasion assay with xCelligence device in MDA-MB-231 cells. MDA-MB-231 cells were pre-treated with VNLG-152R (2.5, 5, and 10 µM) for 24 h and 75 × 10³ cells were plated into 16 well matrigel coated CIM plate in the xcelligence device which measured invasion of cells across the filter every 3 min using electrical impedance across microelectric sensors. The rate of invasion is expressed as the cell index (CI) or the change in electrical impedance at each time point. Values are expressed as the ±SEM of the duplicate wells from three independent experiments. (B) The effects of VNLG-152R on the expression of EMT-related proteins in MDA-MB-231 and MDA-MB-468 cells. (C) About 1 × 10⁶ MDA-MB-231-Luc cells were intravenously injected into a tail vein of SCID mice. VNLG-152R (20 mg/kg, IP) was administered to mice, 5 days/week for 2 weeks. The luciferase signals in the mice were detected and photographed using an IVIS in vivo image system on days 1, 7 and 14.