Table 1.
Chromatographic and analytical parameters for mycotoxin detection by GC-MS/MS: retention time (Rt); quantification (Q) and confirmation (q) transitions; collision energy (CE); dwell time (Dt); limits of detection (LOD) and quantification (LOQ); recovery at three spiked concentration levels; interday relative standard deviation (RSD); matrix effect (ME); linearity expressed as a correlation coefficient (r2).
| Analyte | Rt (min) | SRM (Selection Reaction Monitoring) Transitions (m/z) | CE (eV) | Dt (ms) | LOD (μg kg−1) |
LOQ (μg kg−1) |
Recovery (RSD) (%) | ME ± RSD (%) | Linearity (r2) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LOQ | 2 LOQ | 10 LOQ | ||||||||||||
| DON | 8.45 | 392 > 259 Q | 10 | 25 | 0.5 | 1 | 100 | (7) | 95 | (1) | 127 | (2) | 85 ± 9 | 0.994 |
| 407 > 197 q | 10 | 25 | ||||||||||||
| 3AcDON | 9.45 | 392 > 287 Q | 10 | 25 | 1.25 | 2.5 | 84 | (17) | 85 | (4) | 117 | (15) | 133 ± 7 | 0.995 |
| 467 > 147 q | 5 | 35 | ||||||||||||
| 15AcDON | 9.65 | 392 > 217 Q | 20 | 35 | 2.5 | 5 | 87 | (1) | 82 | (6) | 108 | (11) | 102 ± 6 | 0.996 |
| 392 > 184 q | 20 | 35 | ||||||||||||
| FUS-X | 9.55 | 450 > 26 Q | 10 | 35 | 2.5 | 5 | 79 | (4) | 73 | (3) | 97 | (7) | 137 ± 4 | 0.998 |
| 450 > 245 q | 20 | 35 | ||||||||||||
| DAS | 9.56 | 350 > 229 Q | 15 | 35 | 7.5 | 15 | 83 | (16) | 77 | (11) | 118 | (10) | 66 ± 6 | 0.989 |
| 378 > 124 q | 10 | 25 | ||||||||||||
| NIV | 9.90 | 289 > 73 Q | 15 | 35 | 10 | 20 | 78 | (3) | 88 | (1) | 117 | (5) | 97 ± 9 | 0.994 |
| 379 > 73 q | 15 | 35 | ||||||||||||
| NEO | 11.30 | 252 > 195 Q | 10 | 25 | 10 | 20 | 75 | (4) | 80 | (1) | 86 | (9) | 129 ± 3 | 0.999 |
| 252 > 167 q | 15 | 35 | ||||||||||||
| HT-2 | 14.40 | 347 > 185 Q | 10 | 25 | 7.5 | 15 | 100 | (19) | 84 | (5) | 84 | (7) | 111 ± 6 | 0.989 |
| 347 > 157 q | 10 | 25 | ||||||||||||
| T-2 | 14.45 | 350 > 229 Q | 10 | 25 | 2.5 | 5 | 103 | (1) | 107 | (1) | 113 | (5) | 101 ± 1 | 0.989 |
| 350 > 259 q | 15 | 35 | ||||||||||||
| ZEN | 15.46 | 462 > 151 Q | 20 | 25 | 5 | 10 | 69 | (8) | 106 | (8) | 97 | (3) | 64 ± 4 | 0.999 |
| 462 > 333 q | 20 | 25 | ||||||||||||