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. 2019 Mar 15;8(3):248. doi: 10.3390/cells8030248

Figure 5.

Figure 5

Nic-induced NCLL requires intact Golgi complex. (A,B) FIP200KO-MEFs were treated by Nic (10 μM) with BFA (5 μg/mL) (A) and GCA (20 μM) (B) for 6 h, and then, the LC3-II formation was analyzed. (C) FIP200KO-MEFs expressing GFP-LC3 were treated by Nic (2.5 μM) with BFA (5 μg/mL) and GCA (20 μM) for 6 h, and then, the GFP-LC3 puncta formation was assessed. (D) Quantification of GFP-LC3 puncta in (C). (E) FIP200KO-MEFs were treated with Nic (2.5 μM) for 6 h, followed by immunostaining of GM130 and LC3. (F) FIP200KO-MEFs were treated with Nic (10 μM) for 6 h, and then, the expression levels of GM130 and TGN38 were analyzed. CM, complete medium. ** p < 0.005. Bar = 10 μM.