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Timecourse of M3-04 expression. NIH 3T12 fibroblasts were mock-infected (m) or infected with wild-type MHV68 (WT) at MOI 5. At 6, 12, and 18 hpi, total RNA was harvested and northern blot analysis was performed using probe B to detect M3-04 transcript. 18S rRNA on the methylene blue stained membrane is shown as the loading control.
