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M3-04 is polyadenylated. Poly(A)-selected or total RNA was harvested at 18 hours from either mock-infected (m) or wild-type MHV68-infected (WT) NIH 3T12 fibroblasts. Northern blot was performed using probe B to detect M3-04 transcript. TMER4 and β-actin transcripts were detected using strand-specific probes. Methylene blue staining is shown for rRNA as a loading and poly(A) selection control.
