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M3-04 is a nuclear transcript. Total RNA was harvested at 16 hours from mock-infected (m) or wild-type MHV68-infected (WT) NIH 3T12 fibroblasts, and cytoplasm and nuclear fractions were obtained. Northern blots were probed with strand-specific M3/M2 locus probes B or C, or with probe to the control nuclear host RNA Malat1. Methylene blue staining is shown for rRNA as a loading control.
