Figure 6.

c-MET-mediated RAD51 phosphorylation promotes the formation of high-molecular-weight RAD51 oligomers. (A) The RAD51 WT protein (10 µM) was purified and incubated with ATP (2 mM) and with or without kinase-active c-MET (0.1 µM) and PHA665752 inhibitor (1 µM). Then, the proteins were crosslinked with BS3, separated by SDS-PAGE 10% gel and analyzed by western blot using anti-RAD51 antibodies. The signals were quantified by Odyssey scanner. (B) The graph represents the average of the ratios of the polymer forms of RAD51 phosphorylated or not by c-MET. The ratios were obtained after quantification of the blots from three independent experiments (n = 3; error bars: s.d.; ** p < 0.01).