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. 2019 Mar 13;11(3):355. doi: 10.3390/cancers11030355

Figure 4.

Figure 4

Dynamics of γ-H2AX formation in U2OS, H1299, and HFF-1 cells following treatment with drug combinations. (A) Typical patterns of γ-H2AX staining observed during the time point analysis of U2OS cells by immunofluorescence with mAb 3F4 after treatment with either G, V, A, or G+V are shown. The corresponding cells after DAPI staining are shown in Supplementary Figure S3. Magnification: 630×. (B) U2OS, H1299, and HFF-1 cells were treated with the indicated drugs or drug combinations. At 12 h, 24 h, 48 h, and 72 h post-treatment, the nuclei were stained with mAb 3F4 and the γ-H2AX patterns were scored as indicated in the legend of Figure 3. A minimum of 120 cells from three independent experiments were analyzed in each condition. #, only a fraction of the cells within the analyzed population showed the indicated pattern.