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. 2019 Mar 8;11(3):339. doi: 10.3390/cancers11030339

Figure 1.

Figure 1

Silencing Cx43 enhances the proliferation rate of S1 cells under 2-D and 3-D culture conditions. S1 and Cx43-shRNA S1 cells (Cx43 KO) were cultured under 2-D (A) or 3-D conditions (B). Proliferation rate was assessed by cell counting on days 6 and 10 in 2-D (A; upper panel) and by measurement of acinar diameter on days 4, 6, 9, and 11 in 3-D (B; right panel). An ocular micrometer calibrated against a stage micrometer was used, and acinar areas were then calculated and plotted as acinar size. Fifty acini were analyzed per group. The values depicted in histograms are the means (±S.D.) of cell counts (A; upper panel) or acinar sizes (B; right panel) from three independent experiments. Unpaired t-test; * p < 0.05, ** p < 0.01, *** p < 0.001. Representative images of cells on day 11 in 2-D (A; lower panel) and in 3-D (B; left panel) are shown. Nuclei were stained with Hoechst (blue; B; left lower panel).