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Cx43 assembles GJ complexes in S1 cells under 2-D culture conditions. S1 cells were cultured under 2-D conditions. Total proteins were extracted on day 6. Association of Cx43 and β-catenin (upper panel) or ZO-2 (lower panel) was assessed by coimmunoprecipitation (IP) of Cx43 followed by Western blotting for detection of Cx43, β-catenin and ZO-2. The input served as a control. Three independent experiments were performed for Cx43-β-catenin association and two for Cx43-ZO-2 association.
