FIG. 3.

Patterning mutations alter the dorsolateral eggshell appendage (DA) primordia. Stage 10 egg chambers express Broad (green) and rhomboid-lacZ (magenta) to mark the roof and floor cells, respectively. Anterior is to the left. A white line labels the dorsal midline. (A) Wild type. The complete left primordium and part of the right primordium are visible. (B) A partial loss-of-function mutation in Ras85D disrupts epidermal growth factor signaling and depletes dorsal cell fates. The floor cells form one continuous row anterior to the roof cells. Such egg chambers produce eggshells with a single DA. (C) Over-expression of Decapentaplegic pushes the DA primordia toward the posterior.