Plasma miRNA levels are altered in severe TBI patients. RNA was purified from plasma samples collected within the first 24 h after injury, and assayed by TaqMan qRT-PCR. Dot histogram plots of the ΔCT values for (A) miR-16, (B) miR-92a, (C) miR-765, and (D) area under the curve (AUC) values obtained from receiver operating characteristic (ROC) analysis for each miRNA are shown (healthy volunteer [HV] n = 8; severe TBI [sTBI] n = 7; miRNA, microRNA; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; TBI, traumatic brain injury).