FIG. 2.

Quantification of total lymphocytes and immunological cell subsets in ADA-SCID KO and WT mice over time. The relative lymphocyte populations of KO ADA-SCID mice (left panel) compared with their WT littermates (right panel) are shown over the course of 120 days. The mice were age-matched and followed from 4 months to 8 months old. The lymphocyte counts were obtained from CBC analyses and multiplied by lymphocyte subset percentages, derived from flow cytometry, to yield counts of each lymphocyte subset. Overall, even with substantial SDs, the data suggest that the total lymphocyte, B cell, CD3⁺ cell, CD4⁺ cell, and CD8⁺ cell counts of the KO mice are substantially lower than those of the WT controls. Statistically, when all counts of immunological cells derived from the KO mice, over all time points, are compared with those of the WT mice, over all time points, significant differences with p values <0.05 were observed for total lymphocytes and all cell subsets with the exception of NK cells: total lymphocytes, p < 0.0001; B cells, p < 0.0001; CD3+ cells, p < 0.0001; CD4+ cells, p = 0.0003; CD8, p < 0.0001; NK cells p = 0.8875.