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. 2011 Aug 1;22(8):935–949. doi: 10.1089/hum.2010.121

FIG. 4.

FIG. 4.

Immunohistochemical staining for hADA and staining by hematoxylin and eosin of murine heart and murine kidney, on day 120, following intravenous injection of rAAV9-hADA or saline. (A and B) Representative images of immunohistochemical staining for hADA in cardiac muscle tissue of (A) vector-treated mouse ID #30 and (B) PBS-injected KO mouse ID #27. (C) Hematoxylin and eosin staining of the cardiac muscle tissue of vector-treated mouse ID #30. Overall, a substantial degree of hADA staining was observed throughout cardiac muscle tissue in treated versus untreated KO mice. No inflammatory infiltrates were observed upon hematoxylin and eosin staining of cardiac muscle, which would indicate a host immune response to vector or transgene. (D and E) Representative images of immunohistochemical staining for hADA in the kidney (renal medulla and pelvis) of (D) vector-treated mouse ID #40 and (E) PBS-treated mouse ID #27. (F) Representative image of hematoxylin and eosin staining in the kidney (renal medulla) of vector-treated mouse ID #40. Overall, staining for hADA revealed substantial protein expression in the renal medulla of at least one vector-treated KO mouse compared with untreated KO mice. No inflammatory infiltrate was observed upon hematoxylin and eosin staining, indicating no inflammatory response to vector or transgene was present.