Figure 4.

Lysosomal alkalinization and enlargement caused by nano‐C60 and KN‐93 combination treatment. A) Representative fluorescence images of 143B‐RFP‐Lamp1 cells treated with or without 1.6 µg mL⁻¹ of nano‐C60 in the presence or absence of 10.0 × 10⁻⁶ m KN‐93 for 16 h. Nuclei were stained with DAPI (blue). Scale bar = 20.0 µm. B) Representative fluorescence images of lysosome acidity using LysoSensor Green DND‐189 staining in 143B‐RFP‐Lamp1 cells treated with or without 1.0 µg mL⁻¹ of nano‐C60 in the presence or absence of 7.5 × 10⁻⁶ m KN‐93 for 12 h. Scale bar = 50.0 µm. C) Flow cytometric analysis of lysosome acidity in 143B cells treated as described in B).