Figure 5.

Impaired lysosomal degradation capacity caused by nano‐C60 and KN‐93 combination treatment. A) DQ‐BSA analysis of lysosomal proteolytic activity in 143B cells treated with or without 1.0 µg mL⁻¹ of nano‐C60 in the presence or absence of 7.5 × 10⁻⁶ m KN‐93 for 12 h. The average number of the red fluorescent fragments in each cell was quantified (lower panel). At least 60 cells were analyzed for each treatment. Mean ± SEM, n = 3. **P < 0.01 ***P < 0.005. Scale bar = 50.0 µm. B) and C) Western blot analysis of enzymatic activity of cathepsin B and cathepsin D in 143B and MG63 cells treated with or without 1.6 µg mL⁻¹ of nano‐C60 in the presence or absence of 10.0 × 10⁻⁶ m KN‐93 for 24 h.