Figure 7.

Enhanced synergistic anti‐OS efficacy of nano‐C60 by CaMKIIα inhibitor KN‐93 in vivo. A) Tumors from nude mice treated with PBS (control), 0.5 mg kg⁻¹ KN‐93 (s.c.), 0.2 mg kg⁻¹ nano‐C60 (s.c.), or 0.5 mg kg⁻¹ KN‐93 plus 0.2 mg kg⁻¹ nano‐C60 (s.c.). B) Tumor volume and C) tumor weight in each group are shown. Mean ± SEM, n = 5. **P < 0.01, ***P < 0.005. D) TUNEL staining (green) of tumor tissues was performed to detect apoptotic cells, and nuclei were treated with DAPI (blue). Scale bar = 50.0 µm. E) Western blot analysis of the levels of phospho‐ or total‐CaMKIIα, and LC3 in tumor tissues.