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MΦ-QVOA. Monocytes from blood and macrophages from brain were collected from SIV-infected animals and purified by CD11b-specific bead selection. Macrophages expressing CD11b were plated in serial dilutions in triplicate wells. Cells were cultured with zidovudine (AZT) and darunavir (DRV). Nonadherent cells and the antiretrovirals were removed prior to activation with TNF and co-culture with CEMx174 cells (85, 94).
