Table 2.
Hemolytic activity of G. caribaeus isolates from the eastern and western Caribbean Gulf of Mexico (EC/EGOM and WC/WGOM) and G. carolinianus from the EC/EGOM, WC/WGOM, and patch reefs of the coast of North Carolina, USA (NC). The isolates which were selected for the highest and lowest toxicities in a region based on the initial screening of all 56 isolates (Fig. 4). Cultures were grown at 20, 24, 27, and 31 °C. The resulting EC50 values were normalized on a per cell basis. At each temperature, the EC50 values were measured while the cells were in log, late log – early stationary, and in mid-stationary phase growth (Fig. S1).
| Temperature | Region | Isolate | EC50 cells log phase | EC50 cells late log – early stationary phase | EC50 cells mid-stationary phase |
| Gambierdiscus carolinianus | |||||
| 20 °C | EC/EGOM | Mex Algae gam 1 | 201 | 179 | 222 |
| EC/EGOM | WBHR21 Gam 2 | 284 | 266 | 301 | |
| WC/WGOM | RROV5 | 1799 | 1584 | 1816 | |
| WC/WGOM | ST 1 F7 | 255 | 209 | 281 | |
| NC | Kenny6 | 12205 | 11999 | 12596 | |
| NC | Big Fish gam 5 | 11856 | 11663 | 12001 | |
| 24 °C | EC/EGOM | Mex Algae gam 1 | 189 | 162 | 243 |
| EC/EGOM | WBHR 21 Gam 2 | 259 | 178 | 279 | |
| WC/WGOM | RROV5 | 1672 | 1434 | 1737 | |
| WC/WGOM | ST 1 F7 | 201 | 132 | 269 | |
| NC | Kenny6 | 11887 | 10059 | 12333 | |
| NC | Big Fish gam 5 | 11553 | 11074 | 12887 | |
| 27 °C | EC/EGOM | Mex Algae gam 1 | 149 | 110 | 189 |
| EC/EGOM | WBHR 21 Gam 2 | 236 | 167 | 257 | |
| WC/WGOM | RROV5 | 1587 | 1352 | 1605 | |
| WC/WGOM | ST 1 F7 | 113 | 58 | 236 | |
| NC | Kenny6 | 11212 | 10212 | 12000 | |
| NC | Big Fish gam 5 | 12658 | 12002 | 12888 | |
| 31 °C | EC/EGOM | Mex Algae gam 1 | 181 | 134 | 249 |
| EC/EGOM | WBHR 21 Gam 2 | 175 | 153 | 184 | |
| WC/WGOM | RROV5 | 1331 | 1183 | 1549 | |
| WC/WGOM | ST 1 F7 | 99 | 77 | 123 | |
| NC | Kenny6 | 10008 | 9608 | 12750 | |
| NC | Big Fish gam 5 | 9902 | 9810 | 10100 | |
| Temperature | Region | Isolate | EC50 cells Log | EC50 cells Late log | EC50 cells Stationary |
| Gambierdiscus caribaeus | |||||
| 20 °C | EC/EGOM | Mexico Algae 1 gam 1 | 212 | 206 | 294 |
| EC/EGOM | WBHR 26 gam 1 | 208 | 198 | 273 | |
| EC/EGOM | Gam 19 | 234 | 191 | 251 | |
| WC/WGOM | Keys jar 7 gam 7 | 245 | 232 | 265 | |
| WC/WGOM | Dive 1 fa gam 4 | 301 | 274 | 317 | |
| 24 °C | EC/EGOM | Mexico Algae 1 gam 1 | 205 | 181 | 234 |
| EC/EGOM | WBHR 26 gam 1 | 216 | 190 | 259 | |
| EC/EGOM | Gam 19 | 202 | 196 | 238 | |
| WC/WGOM | Keys jar 7 gam 7 | 239 | 221 | 269 | |
| WC/WGOM | Dive 1 fa gam 4 | 273 | 256 | 304 | |
| 27 °C | EC/EGOM | Mexico Algae 1 gam 1 | 209 | 140 | 194 |
| EC/EGOM | WBHR 26 gam 1 | 91 | 69 | 121 | |
| EC/EGOM | Gam 19 | 58 | 16 | 121 | |
| WC/WGOM | Keys jar 7 gam 7 | 246 | 172 | 262 | |
| WC/WGOM | Dive 1 fa gam 4 | 263 | 231 | 284 | |
| 31 °C | EC/EGOM | Mexico Algae 1 gam 1 | 198 | 176 | 267 |
| EC/EGOM | WBHR 26 gam 1 | 94 | 77 | 125 | |
| EC/EGOM | Gam 19 | 64 | 31 | 112 | |
| WC/WGOM | Keys jar 7 gam 7 | 253 | 233 | 331 | |
| WC/WGOM | Dive 1 fa gam 4 | 250 | 229 | 319 | |