Figure 1.

Revalidation of effects of induced cisplatin (CDDP) chemoresistance on phenotypic properties of UKF-NB-4 cells. (A) Phase contrast micrographs showing the morphology changes in UKF-NB-4^CDDP cells. Scale bar, 100 µm. (B) MTT dose-response curves of UKF-NB-4 and UKF-NB-4^CDDP cells exposed to CDDP (1–50.0 µg/mL) for 24 h. (C) Distribution of CDDP-induced apoptosis and living cell counts in both tested cell lines evaluated by flow cytometry. The data are results from three independent experiments. * p < 0.01 related to parental cells. (D) Detailed representative phase contrast micrographs of clonogenic assay plates showing significant differences in survival and clonal efficiency. The length of scale bar is 1000 µm. (E) Quantitation of clonal efficiency of cells exposed to CDDP. The data are results from three independent experiments. * p < 0.05 related to control non-treated cells.