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. 2019 Mar 12;8(3):235. doi: 10.3390/cells8030235

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Re-sensitizing of UKF-NB-4^CDDP cells by lysosomotropic agents chloroquine (CQ) and Baf. (A) Whole-cell lysates immunoblots demonstrating conversion of LC3-I to LC3-II as a result inhibition of autophagy causing intracellular accumulation of autophagosomes. β-Actin–loading control. (B) Trypan blue exclusion data showing the re-sensitizing activity of CQ and Baf in UKF-NB-4^CDDP cells. The data are results from five independent experiments. * p < 0.01 related to CDDP cytotoxicity. +/− indicates the presence of CDDP, CQ or Baf in the treatment.