Fig. 1.

Schema illustrating procedural steps to generate MSCs and expansion in flasks to characterize cells. Heparinized bone marrow aspirate was delivered within 24 h of extraction, diluted and seeded in tissue culture flasks using MSC growth media. Following initial wash steps colony formation occurred (P0 generation). To provide a homogenous population for bioreactor expansion, cells were mixed, replated and grown to sub confluence. This was termed as pre-quantum expansion passage 1 (PreQE-P1). These cells were expanded manually and assayed for metabolic activity, cell proliferation, morphology, cell marker expression, and potency