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. 2019 Apr 17;17:128. doi: 10.1186/s12967-019-1877-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Frequency of MSC markers is maintained on QE1, QE2 and manually expanded passage 3 cells. Cryopreserved MSCs from donor Pigs 1, 2, and 3 were thawed and stained with mesenchymal stem cell markers CD44-APC, CD90-PE, and CD105-Alexa405 along with a FITC cocktail containing lineage markers CD45, CD31, and SLA-DR Class II. Cells were pregated to exclude debris and doublets before gating on total live cells. a Frequency of CD90, CD44, and CD105 positive cells within QE1 cells of each donor Pig. b Frequency of CD90, CD44, and CD105 positive cells within QE2 cells for Pigs 1 and 3 and manually expanded Passage 3 cells of Pig 2 grown in tissue culture flasks. c Quantitative assessment of CD90, CD44, and CD105 positive cells within QE2 cells for Pigs 1 and 3 and manually expanded passage 3 cells of Pig 2 grown in tissue culture flasks