Table 1.
List of proteins identified exclusively in ESP from Schistosoma japonicum eggs
| Accession | Protein name | Unused | % Cov | Peptides (95%) | SecP |
|---|---|---|---|---|---|
| tr|Q1HDV2 | Fructose-bisphosphate aldolase | 66.55 | 88.98 | 116 | – |
| tr|B2LXU1 | Enolase | 0.20 | 65.89 | 47 | – |
| tr|C1LRC5 | Carbonyl reductase 1 | 0.00 | 81.95 | 45 | – |
| tr|Q5DFP8 | Putative uncharacterized protein | 2.00 | 75.83 | 43 | – |
| tr|C1LKA0 | Purine nucleoside phosphorylase | 2.17 | 72.13 | 40 | NC |
| tr|G4VHK8 | Tubulin beta chain | 40.95 | 6.24 | 33 | NC |
| tr|C1LIX7 | Loss of heterozygosity 11 chromosomal region 2 gene A protein homolog | 31.78 | 45.39 | 23 | NC |
| tr|C1LIA3 | Dihydrolipoyl dehydrogenase | 36.56 | 57.74 | 22 | NC |
| tr|C1LAW4 | Major egg antigen (P40) | 19.2 | 48.01 | 22 | – |
| tr|A0A183N4E4 | Uncharacterized protein | 0.00 | 43.66 | 19 | SP |
| tr|Q5DBS1 | SJCHGC07012 protein | 29.51 | 51.88 | 18 | NC |
| tr|C1LNQ7 | Protein disulfide-isomerase | 27.32 | 43.20 | 17 | SP |
| tr|Q5DEE6 | Peptidyl-prolyl cis-trans isomerase | 10.54 | 55.48 | 15 | – |
| tr|G4V9B9 | Putative heat-shock protein | 0.03 | 26.78 | 15 | – |
| tr|C1LS07 | Cell wall integrity and stress response component 1 | 0.00 | 51.16 | 15 | – |
| tr|C1L675 | Arginase OS=Schis | 25.96 | 52.20 | 14 | – |
| tr|C1LWQ9 | Elongation factor 1-alpha | 17.02 | 64.45 | 14 | – |
| tr|A0A094ZS46 | Spectrin alpha chain | 25.99 | 33.16 | 12 | – |
| tr|C7TYX3 | Putative uncharacterized protein | 0.00 | 4.30 | 12 | SP |
| tr|A0A183QGT0 | Tubulin alpha chain | 0.00 | 29.92 | 11 | – |
| tr|G4VSJ5 | Putative glycogen phosphorylase | 0.00 | 37.74 | 11 | – |
| tr|Q7Z0T1 | Phosphoglycerate kinase | 15.49 | 49.75 | 9 | – |
| tr|C1LLK8 | Peptidase M8, leishmanolysin,domain-containing protein | 9.56 | 33.91 | 9 | SP |
| tr|C7TQR6 | Ribonuclease X25 | 8.96 | 53.29 | 9 | SP |
| tr|Q5DHF8 | Histone H3 | 5.64 | 41.17 | 9 | NC |
| tr|C1L4U6 | 3’(2’), 5’-bisphosphate nucleotidase | 16.83 | 36.62 | 8 | SP |
| tr|C1LYD5 | Ubiquitin C | 7.32 | 7.80 | 8 | – |
| tr|C1LQN5 | Uncharacterized protein | 0.08 | 44.90 | 8 | SP |
| tr|A0A094ZSL4 | Spectrin beta chain | 13.2 | 21.92 | 7 | – |
| tr|C1L6J2 | Uncharacterized protein | 12.2 | 47.83 | 7 | SP |
| tr|Q86DZ2 | Clone ZZZ395 mRNA sequence | 10.59 | 39.91 | 7 | – |
| tr|C1LEJ4 | Histone H2A | 5.28 | 56.80 | 7 | NC |
| tr|C1LUP9 | Peptidase inhibitor 16 | 0.00 | 46.02 | 7 | SP |
| tr|C1L9E0 | Receptor expression-enhancing protein | 8.30 | 35.44 | 6 | NC |
| tr|G4VS58 | Putative prohibitin | 7.97 | 34.02 | 6 | NC |
| tr|A0A183L8B7 | Uncharacterized protein | 1.70 | 35.49 | 6 | NC |
| tr|G4VG96 | Cell polarity protein | 10.72 | 18.52 | 5 | – |
| tr|Q5DAE9 | SJCHGC09095 protein | 8.60 | 43.95 | 5 | – |
| tr|C1LRF9 | Fatty acid binding protein 7, brain | 8.27 | 80.29 | 5 | – |
| tr|Q7JNB4 | Aspartic protease (Fragment) | 6.14 | 27.34 | 5 | SP |
| tr|A0A183MKN4 | Uncharacterized protein | 5.08 | 3.04 | 5 | – |
| tr|Q5DF47 | Putative uncharacterized protein | 8.77 | 41.81 | 4 | – |
| tr|A0A183NI42 | Uncharacterized protein | 7.00 | 31.92 | 4 | – |
| tr|G4VFV4 | Putative rap1 | 4.97 | 30.97 | 4 | NC |
| tr|A0A183PY58 | Uncharacterized protein | 4.02 | 15.36 | 4 | NC |
| tr|A0A183NZF1 | Peptidyl-prolyl cis-trans isomerase | 0.07 | 30.52 | 4 | SP |
| tr|C1LID9 | LAMA-like protein 2 | 6.72 | 1.90 | 3 | SP |
| tr|C1LA47 | Cathepsin B-like cysteine proteinase | 6.18 | 25.15 | 3 | SP |
| tr|Q5C774 | SJCHGC02069 protein (Fragment) | 5.38 | 3.84 | 3 | SP |
| tr|Q5D9E0 | SJCHGC01621 protein | 4.86 | 2.87 | 3 | NC |
| tr|C1LCS9 | Phosphatase 2A inhibitor I2PP2A | 4.86 | 52.49 | 3 | – |
| tr|C1LKV5 | Rho GDP-dissociation inhibitor 2 | 4.85 | 45.73 | 3 | – |
| tr|Q5DBC3 | SJCHGC06819 protein | 4.57 | 17.52 | 3 | NC |
| tr|Q5DFY2 | SJCHGC06929 protein | 4.40 | 25.61 | 3 | SP |
| tr|Q5DFR4 | ATP:ADP antiporter | 4.01 | 40.83 | 3 | – |
| tr|A0A183QZZ1 | Uncharacterized protein | 4.00 | 19.41 | 3 | NC |
| tr|Q8I8A1 | Rho2 GTPase (Fragment) | 3.82 | 32.80 | 3 | NC |
| tr|Q03528 | 22.6kd tegumental associated antigen | 3.75 | 35.60 | 3 | NC |
| tr|G4VDJ6 | Putative adp-ribosylation factor, arf | 0.06 | 45.86 | 3 | NC |
| tr|A0A183NLB0 | T-complex protein 1 subunit gamma | 0.03 | 24.59 | 3 | – |
| tr|C1LNJ0 | Putative Retinol dehydrogenase 11 | 4.92 | 39.01 | 2 | NC |
| tr|A0A183K1U2 | Uncharacterized protein | 4.81 | 19.40 | 2 | SP |
| tr|A0A183JQD4 | Uncharacterized protein | 4.48 | 31.16 | 2 | – |
| tr|C7TUC3 | Putative uncharacterized protein | 4.05 | 71.74 | 2 | SP |
| tr|G4V9G5 | Putative 60s ribosomal protein L12 | 4.03 | 45.44 | 2 | NC |
| tr|C1L7G5 | Ferritin | 4.00 | 31.97 | 2 | – |
| tr|Q5BT38 | SJCHGC02867 protein (Fragment) | 3.73 | 21.87 | 2 | – |
| tr|C1LTL1 | Universal stress protein | 3.65 | 35.22 | 2 | – |
| tr|C1LKU6 | Erythrocyte band 7 integral membrane protein | 3.24 | 15.64 | 2 | NC |
| tr|C1LNJ7 | Egg protein CP1531 | 3.18 | 17.52 | 2 | SP |
| tr|G4V7S8 | Serine hydroxymethyltransferase | 3.09 | 69.43 | 2 | – |
| tr|C1L857 | Chaperonin containing TCP1, subunit 5 (Epsilon) | 2.98 | 35.98 | 2 | – |
| tr|A0A094ZXB9 | Aminoacylase-1 | 2.89 | 17.02 | 2 | – |
| tr|Q9B8Z2 | Cytochrome c oxidase subunit 2 | 2.82 | 84.16 | 2 | SP |
| tr|G4VJ08 | Acyl-CoA thioesterase-related | 2.47 | 10.39 | 2 | NC |
| tr|C1LEX9 | Transmembrane protease, serine 6 | 2.21 | 13.34 | 2 | SP |
| tr|G4M1G4 | REVERSED Dynein heavy chain, putative | 2.14 | 57.13 | 2 | – |
| tr|A0A183P199 | Uncharacterized protein | 2.06 | 13.37 | 2 | NC |
| tr|A0A0R4I956 | Myosin 2 heavy chain | 2.05 | 15.50 | 2 | – |
| tr|G4VB27 | Putative uncharacterized protein | 2.02 | 95.39 | 2 | – |
| tr|G4V6J0 | Putative copine | 2.02 | 56.00 | 2 | NC |
| tr|A0A094ZYC9 | Dolichyl-diphosphooligosaccharide–protein glycosyltransferase subunit 2 | 2.01 | 9.40 | 2 | SP |
| tr|C1LP66 | Aquaporin-3 (AQP-3) | 2.00 | 15.24 | 2 | – |
| tr|A0A183LXE3 | Uncharacterized protein | 2.00 | 32.67 | 2 | NC |
| tr|G4LXF1 | Rab6, putative | 1.87 | 33.98 | 2 | NC |
| tr|A0A183KN75 | Uncharacterized protein | 1.68 | 17.46 | 2 | NC |
| tr|Q7Z1I7 | GTP-binding protein-like protein | 1.37 | 14.06 | 2 | – |
| tr|A0A183NZ41 | Uncharacterized protein | 1.37 | 79.14 | 2 | – |
| tr|Q5DI05 | Elongation factor Tu | 1.24 | 16.21 | 2 | SP |
| tr|A0A183MXQ1 | Uncharacterized protein | 1.24 | 27.05 | 2 | NC |
| tr|A0A183QCJ7 | Uncharacterized protein | 1.16 | 95.11 | 2 | NI |
| tr|A0A183LI65 | Uncharacterized protein | 0.77 | 19.61 | 2 | – |
| tr|Q7Z1I5 | 40S ribosomal protein S8 | 0.64 | 23.57 | 2 | – |
| tr|G4VQJ4 | Putative adenine phosphoribosyltransferase | 0.09 | 96.77 | 2 | – |
| tr|A0A095ACH1 | REVERSED Uncharacterized protein (Fragment) | 0.00 | 24.43 | 2 | NC |
Abbreviations: ESP, egg secretory proteins; Unused, quantification for proteins in the ProteinPilot software; %Cov, ratio of the protein sequence covered by the matched peptides; Peptides (95%) total number of detected peptides with 95% of confidence; SecP, (Secretome P 2.0) results described as: SP, indicate presence of predicted signal sequence; NC, indicate non-classical secreted proteins; (–), non-detected