Fig. 4.

Activity and solubility of mutants of 7421ADO. a The amount of total hydrocarbon produced in E. coli coexpressing 7942AAR and a mutant of 7421ADO relative to that of wild-type (WT) 7421ADO. b The hydrocarbon-producing activity of a 7421ADO mutant relative to that of the WT. c Fractions of pentadecane, heptadecene, and heptadecane relative to the total amount of hydrocarbon produced by a 7421ADO mutant. d The amount of the soluble form of a 7421ADO mutant relative to that of the WT. e Solubility (%) of ADO. f The protein expression level of 7421ADO relative to that of the WT. In all panels, the data are shown in descending order of activity. A horizontal dotted line shows the value for the WT. The measurements were carried out more than three times, and the means ± standard errors are shown