Fig. 3.

Inhibiting endogenous lncSBF2-AS1 in recurrent GBM cells promotes TMZ-induced cell apoptosis (a) SBF2-AS1 knockdown lentivirus transfected Rec GBM and N3T3rd cells showing reduced SBF2-AS1 expression. Data represents means of three independent experiments ± SEM. ***P < 0.001. b Colony formation ability of SBF2-AS1 knockdown cells or vector control cells in the absence or presence of TMZ. Data represents means of three independent experiments ± SEM. ***P < 0.001. c Flow cytometry was used to measure the apoptosis of SBF2-AS1 knockdown cells or vector control cells after exposure to 200 μM TMZ for 48 h. Data represents means of three independent experiments ± SEM. ***P < 0.001. d Western blot test of cleaved caspase-3 expression of SBF2-AS1 knockdown cells or vector control cells in the absence or presence of TMZ. Tubulin was used as the loading control. e TUNEL analysis of SBF2-AS1 knockdown cells or vector control cells in the absence or presence of TMZ for 48 h. Data represents means of three independent experiments ± SEM. ***P < 0.001. Scale bar, 50 μm. f Representative images of Rec GBM SBF2-AS1-knockdown or shctrl cells-derived subcutaneous tumors on 34th day in the absence or presence of TMZ. g Growth curve of tumor xenografts originated from Rec GBM SBF2-AS1-knockdown or shctrl cells in the absence or presence of TMZ. Data represents means of three independent experiments ± SEM. *P < 0.05, ***P < 0.001. h Tumor weight of tumor xenografts originated from Rec GBM SBF2-AS1-knockdown or shctrl cells in the absence or presence of TMZ. Data represents means of three independent experiments ± SEM. **P < 0.01. i IHC analysis of cleaved caspase-3 in Rec GBM SBF2-AS1-knockdown or shctrl cells-derived subcutaneous tumors in the absence or presence of TMZ. Scale bar, 50 μm