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. 2019 Mar 26;12(1):45. doi: 10.3390/ph12010045

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemistry (IHC) for APELA protein expression in BTICs and differentiated BTICs. IHC was performed on bulk differentiated and X10 and X16 BTICs. Staining was performed with APELA antisera or pre-immune sera and processed for IHC. Nuclei were counterstained with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI). Note, the markedly more intense cytoplasmic staining for APELA in BTICs than in bulk GBM cells, and no detectable staining obtained with pre-immune sera. Representative images are shown. Staining with pre-immune sera showed no IHC staining.