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Typical chromatogram of the semi-preparative RP-HPLC purification of the crude mixture: The [11C]mHED elutes after 9.5 min from the C-18 RP column after using a step gradient, as illustrated at the top of the graphic. Solvent A consists of an acetonitrile:water mixture (58:42) and solvent B is a mixture of acetonitrile and acidified water (50:50; 0.004% H3PO4, conc).
