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. Author manuscript; available in PMC: 2019 Apr 17.
Published in final edited form as: Mol Pharm. 2018 Oct 11;15(11):5135–5145. doi: 10.1021/acs.molpharmaceut.8b00707

Figure 6.

Figure 6.

Gli1 PEI–SNAs reduce stemness and impair self-renewal of U87 cells. (A) Schematic depicting the neurosphere culture model and experimental design; red cells illustrate GSCs. (B) qPCR showing expression of genes associated with stemness following exposure to PEI–SNAs. Gene expression is normalized to that of GAPDH. Data are means ± STDs; *p < 0.001 relative to Scr PEI–SNA. (C) Representative bright-field images of neurospheres cultured from U87 cells after exposure to PEI–SNAs. Scale = 200 μm. (D) Gli1 PEI–SNAs reduce the size and number of neurospheres formed, as measured from 25 tiled bright-field images per treatment group per experiment; *p = 0.03 by Student’s t-test.