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| Gene locus | Real-time PCRa | Fluorescent microscopyb |
|---|---|---|
| TTC1610 (oriC region) | 7.87 ± 0.73 | 7.53 ± 2.71 |
| TTC0574 (relatively closer to terC) | 7.01 ± 1.55 | / |
real-time PCR method was used to measure the chromosome copy numbers.
Fluorescent microscopy was used to count ParB-sGFP fluorescent foci numbers per cell reflecting the copy numbers of the chromosomal origin region (100 cells were counted).
