Table 1. Summary of candidates identified in screen of RNAi knockdowns.

Stock ID#	Gene name	Protein Function	% Penetrance Male	% Penetrance Female	% Penetrance Total	Phenotype
Calcium Channels
BL27263	Stim	CRAC channel regulator	100	100	100	Wings small and malformed, thick veins, blisters
BL31295	nan	Transient receptor potential channel*	84	32	54	L5 incomplete, L5 bifurcation
BL31292	wtrw	Transient receptor potential channel*	69	39	53	PCV incomplete
Sodium Channels
BL25847	rpk	DEG/epithial sodium channel*	100	85	92	L5 incomplete or bifurcation, L4 bifurcation
BL27088	ppk25	DEG/epithial sodium channel*	22	37	30	PCV bifurcation, L5 bifurcation
BL25810	ppk30	DEG/epithial sodium channel*	73	30	55	PCV incomplete, L4 & L5 bifurcations, L5 incomplete,
Potassium Channels
VDRC 28430	Irk1	Inwardly rectifying K+ channel	100	91	95	L5 & L4 bifurcations, loss of ACV, thick veins
VDRC 4341	Irk2	Inwardly rectifying K+ channel	93	85	89	L5 & L4 bifurcations, loss of ACV, thick veins
VDRC 3886	Irk3	Inwardly rectifying K+ channel	100	30	47	L5 & L4 bifurcations, loss of ACV, thick veins
Chloride Channels
BL42654	Best2	Calcium activated chloride channel*	100	100	100	Wings small and severely malformed
BL39040	Best3	Chloride channel*	100	40	66	Small narrow wings (male), missing ACV, L2 bifurcation, PCV incomplete
Ligand-gated Cation Channels
BL62391	Ir7b	Ionotropic receptor*	86	97	92	PCV incomplete or bifurcation, L5 bifurcation
BL34678	Ir76a	Ionotropic receptor	97	46	74	PCV incomplete, L5 bifurcation
BL53975	Ir94h	Ionotropic receptor*	24	29	27	Bristle defects
Other
BL30501	Inx3	Gap junction channel	98	38	68	PCV incomplete, L4 bifurcation, L5 bifurcation, L5 incomplete

At least 20 female and 20 male flies were scored for each line.

BL, Bloomington Drosophila Stock Center number, VDRC, Vienna Drosophila Resource Center number.

PCV, posterior cross vein, ACV, anterior cross vein, L, longitudinal vein.

^*Function predicted by sequence similarity.