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. 2019 Apr 10;10:765. doi: 10.3389/fimmu.2019.00765

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Copyright © 2019 Sierra Parraga, Rozenberg, Eijken, Leuvenink, Hunter, Merino, Moers, Møller, Ploeg, Baan, Jespersen and Hoogduijn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Perfusion fluid and thawing after cryopreservation increase ROS production and reduce metabolic activity in MSC. (A) ROS production in fresh and frozen-thawed pMSC suspended in culture medium or in perfusion fluid. (B) ROS production in fresh and in frozen-thawed hMSC suspended in culture medium or in perfusion fluid. (C) Metabolic activity measured by XTT reduction by NADH of fresh and frozen-thawed pMSC after 30 min and 2 h incubation in perfusion fluid. (D) Metabolic activity measured by XTT reduction by NADH of fresh and frozen-thawed hMSC after 30 min and 2 h incubation in perfusion fluid (n = 5). Results are shown as means ± SD. *p < 0.05; **p < 0.01.