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. 2019 Apr 10;10:765. doi: 10.3389/fimmu.2019.00765

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Copyright © 2019 Sierra Parraga, Rozenberg, Eijken, Leuvenink, Hunter, Merino, Moers, Møller, Ploeg, Baan, Jespersen and Hoogduijn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of perfusion fluid on attached MSC. Survival of attached pMSC (A) and hMSC (B) after 30 min in perfusion fluid. (C,D) Metabolic activity of attached pMSC (C) and hMSC (D) after 30 min in perfusion fluid measured by reduction of XTT. (E,F) Proliferation of attached pMSC (E) and hMSC (F) after 30 in perfusion fluid. Cells were trypsinized and re-seeded in a culture flask. Proliferation after 24 h was determined by CFSE fluorescence (n = 5). Results are shown as means ± SD. *p < 0.05; **p < 0.01.