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The scrape-loading assay (20) was used to evaluate levels of gap junction-mediated intercellular communication in AL-AH1-9-transfected cells. In connexin 43 overexpressing (CX10) cells (A), Lucifer yellow migrated a distance of several cell layers away from the scrape. In contrast, in cells transfected with the dominant negative vector (DN6) cells (B), there was no migration of the dye (×200).
