FIG. 1.

UAS-regulated transgenic tools for subcellular labeling. All embryos were generated by crossing transgenic UAS reporter lines with a stable BAC transgenic line expressing Gal4-VP16 under the control of ptf1a regulatory elements.¹²⁸ (A, B) Mitochondrial labeling from a 14 × UAS:cox5a-eGFP fusion in the hindbrain (shown at low power in inset in A). (C) Nuclear localization of 14 × UAS:hmgb1-GFP in hindbrain neurons. (D) Membrane-targeted 14 × UAS:mCherry-CAAX expression outlines the morphology of spinal cord interneurons. (A) and (B) are dorsal views with embryos oriented with anterior to the left, (C) is a dorsal view with anterior to the top, and (D) is a lateral view. Constructs and transgenic lines are available upon request.