FIG. 7.

Effect of AMS on X-ray-induced activation of NF-κB-dependent genes in mouse kidney. Mice were treated as described in Materials and Methods. Kidney homogenates were prepared to determine the levels of VCAM-1, iNOS, and COX-2 using western blot. A representative blot is shown from three independent experiments with identical observations (n = 5), and equivalent protein loading was confirmed by probing for β-actin. Blot density was detected with FluorChem and standardized as a percentage of the untreated group. Results of one-factor ANOVA followed by Fisher's protected LSD post hoc test were used: **P < 0.01, ***P < .001 versus nonexposed mouse kidney; ^##P < .01, ^###P < .001 versus X-ray-exposed mouse kidney, respectively.