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. 2009 May 21;12(2):351–358. doi: 10.1089/jmf.2008.0006

FIG. 1.

FIG. 1.

t-BHP-induced RS generation was suppressed by kaempferol (Kp) in YPEN-1 cells. (A) Quantitative analysis of fluorescence intensity using DCF-DA was detected after treatment with vehicle or 200 μM t-BHP in the absence or presence of 0.008, 0.2, and 5 μM Kp and 50 μM N-acetyllysine (NAC) for 6 hours. Results of one-factor analysis of variance: ***P < .001 versus untreated control; #P < .05, ##P < .01, ###P < .001 versus 200 μM t-BHP-treated group. (B) Intracellular RS were detected by DCF-DA using a fluorescence microscope: untreated control, 200 μM t-BHP treated alone, 200 μM t-BHP and 0.2 μM Kp, and 200 μM t-BHP and 5 μM Kp.