Phylogenetic tree analysis based on the nucleotide sequences of gag p17 (A), env C2V3 (B), and gag p6 (C) regions obtained from various risk groups. Left panels in (A) and (B) include sample numbers 1 to 100 and right panels in (A) and (B) include sample numbers 101 to 214, with reference sequences obtained from the Los Alamos National Laboratory HIV Database (http://hiv-web.lanl.gov/), respectively. (C) consists of five recombinant forms and nine randomly chosen samples. Each color represents risk factor groups. Open rectangular boxes and arrows indicate five recombinant forms with subtype discordance among the gag p17, env C2V3, and gag p6 genes. IDU, intravenous drug use; HET, heterosexual transmission; Mix, subjects with both IDU and heterosexual risk factors; MSM, males having sex with males; MTCT, mother-to-child transmission; Others, other risk factors such as blood transfusion, tattoo, and unknown transmission.