Figure 2. Effect of IL-33 on cytokines production in HCECs stimulated with A. fumigatus.

HCECs were exposed to A. fumigatus hyphae (2, 5, or 10×10⁶) for 0, 4, 8, and 16h with or without prior incubation of recombinant human IL-33 (10 ng/mL). A, C: mRNA expression levels of inflammatory cytokines (IL-1β and IL-6) measured by qRT-PCR; B, D: Protein expression levels of inflammatory cytokines determined by ELISA in culture supernatants. Results shown are mean±SD of four independent experiments. ^aP<0.05; ^bP<0.01, ^cP<0.001, n=4.