Fig. 2.

Assessment of the quantitative impact of deletion of four thioesterase-encoding genes on 3HB production by E. coli AF1000 expressing t3 and rx. Final 3HB concentration was measured at the end of the nitrogen-depleted batch cultivation, whereas the reported specific 3HB productivity (q_3HB) and yield of 3HB on glucose (Y_3HB/Glc) were calculated over the nitrogen-depleted phase. Mean deviations for all strains were calculated from duplicate experiments, except for the control and ΔtesA strains, which were done in triplicate. The control strain was E. coli AF1000 expressing t3 and rx without thioesterase deletions