Table 1.
Strains and plasmids used in this study
| Strain/plasmid | Description/genotype | Source |
|---|---|---|
| AF1000 | MC4100, relA+ | Sandén et al. (2003) |
| AF1000 ∆tesA | AF1000, ∆tesA::FRT | This study |
| AF1000 ∆tesB | AF1000, ∆tesB::FRT | This study |
| AF1000 ∆fadM | AF1000, ∆fadM::FRT | This study |
| AF1000 ∆yciA | AF1000, ∆yciA::FRT | This study |
| DH5 | F− φ80lacZΔM15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17(rK−, mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Invitrogen |
| pJBGT3Rx | t3 and rx from H. boliviensis under placUV5 and lacI control (p15A/Cm) | Jarmander et al. (2015) |
| pBADzwf | zwf from E. coli under control of paraBAD (pBR22/Amp) | Perez-Zabaleta et al. (2016) |
| pJBG-Blank | placUV5 and lacI control (p15A/Cm) | This study |
| pBAD-(Km)-Blank | paraBAD (pBR22/Km) | This study |
| pBAD-(Km)-yciA | yciA from E. coli AF1000 under control of paraBAD (pBR22/Km) | This study |
| pBAD-(Km)-yciA-zwf | yciA and zwf from E. coli AF1000 under control of paraBAD (pBR22/Km) | This study |
| pBAD-(Km)-zwf-yciA | yciA and zwf from E. coli AF1000 under control of paraBAD (pBR22/Km) | This study |
| pBAD-(Km)-zwf | zwf from E. coli AF1000 under control of paraBAD (pBR22/Km) | This study |
| pKD3 | Template plasmid used for the FRT-flanked cat cassette. | Addgene # 45604, Datsenko and Wanner (2000) |
| pCmFRT* | pKD3 plasmid used with removed RBSs. | This study |
| pKD4 | Template plasmid used for the Km cassette | Addgene #45605, Datsenko and Wanner (2000) |
| pSIJ8 | λ red recombinase genes and flippase recombinase expression | Addgene # 68122, Jensen et al. (2015) |