Table 2.

Calculated parameters for 3HB production in a nitrogen-depleted batch by E. coli and different plasmid combinations

Expressed plasmids	Phase	Growth rate	3HB titer	q3HB	Y3HB/Glc
		(h−1)	(g L−1)	(g g−1 h−1)	(g g−1)
pJBG-Blank pBAD-(Km)-Blank	Exp. growth	0.739 ± 0.017	n.d.	–	–
	N-depletion	–	n.d.	–	–
pJBGT3RX pBAD-(Km)-Blank	Exp. growth	0.597 ± 0.010	0.35 ± 0.03	0.102 ± 0.003	0.060 ± 0.003
	N-depletion	–	0.70 ± 0.06	0.045 ± 0.002	0.162 ± 0.010
pJBGT3RX pBAD-(Km)-yciA	Exp. growth	0.521 ± 0.003	0.65 ± 0.03	0.205 ± 0.011	0.120 ± 0.096
	N-depletion	–	1.45 ± 0.07	0.115 ± 0.006	0.302 ± 0.019
pJBGT3RX pBAD-(Km)-zwf-yciA	Exp. growth	0.477 ± 0.004	0.98 ± 0.04	0.270 ± 0.008	0.170 ± 0.012
	N-depletion	–	1.93 ± 0.12	0.153 ± 0.003	0.302 ± 0.007

n.d. no product detected

Mean deviation was calculated for triplicate experiments for all the plasmid combinations except for combination pJBGT3RX pBAD-(Km)-zwf-yciA in which the mean deviation was calculated for duplicate