Table 3.

Oligonucleotide sequences for in situ padlock probe technology

Primer	Sequence (5′–3′)
ACTB*	C+GG+GC+GG+CG+GATCGGCAAAG

Padlock probe	Sequence (5′–3′)
plp_ACTB*	AGCCTCGCCTTTGCCTTCCTTTTACGACCTCAATGCACATGTTTGGCTCCTCTTCGCCCCGCGAGCACAG

Detection probe	Sequence (5′–3′)
D1_ATTO 550	ATTO 448-CCTCAATGCACATGTTTGGCTCC

Padlock probe was 5′-phosphorylated. The fluorophore of the detection oligonucleotide was 5′ conjugated

+: the following base is LNA modified; underlined: target complement sequence, bold: the complementary detection probe sequence