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In vitro ubiquitylation of gp78. (A) In vitro ubiquitylation in the presence of E1, UbcH5B (H5B), 32P-labeled Ub, and 20 pmol of the indicated GST fusion proteins performed as described. (B) In vitro ubiquitylation using the same GST fusion proteins as in A, but MmUBC7 was used instead of UbcH5B. (C) gp78C and its truncated mutants as presented in Fig. 3A were assayed for ubiquitylation in the presence of MmUBC7 (M7) (Upper) or UbcH5B (H5B) (Lower).
