Table 1. The qRT-PCR primers. Sequences (5’-3’) of reverse transcription qRT-PCR specific primers of candidate genes expressed in bovine embryos.
| Gene | Description | Forward and reverse primers (5’→3’) | Product size (bp) |
|---|---|---|---|
| POU5F1 | POU class 1 homeobox 1. Critical for somatic cell reprogramming and ESC self-renewal. |
F:
CGAGAGGATTTTGAGGCTGC R: GAGTACAGTGTGGTGAAGTGAG |
122 |
| SOX2 | SRY (Sex Determining Region Y)-Box 2. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
F:
CTCGCAGACCTACATGAACG R: TGGGAGGAAGAGGAAACCAC |
144 |
| MYC | Myc proto-oncogene protein, bHLH transcription factor. |
F:
GGCTAAGTTGGACAATGGCAG R: TTCAGCTCGTTCCTCCTCTG |
141 |
| KLF4 | Kruppel like factor 4. Critical for somatic cell reprogramming and ESC self-renewal. |
F:
CATCAGCCTCATCCTCGTC R: TTCAGCTCGTTCCTCCTCTG |
148 |
| CDX2 | Caudal Type Homeobox 2. Trophectoderm differentiation. |
F:
AACCGCAGAGCAAAGGAAAG R: AGGGAAGACACAGGACTCAG |
143 |
| GAPDH | Glyceraldehyde-3-Phosphate dehydrogenase. Reference gene. |
F:
GCTGAGTACGTTGTGGAGTC R: TCACGCCCATCACAAACATG |
133 |